Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility.
Identifieur interne : 000F08 ( Main/Exploration ); précédent : 000F07; suivant : 000F09Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility.
Auteurs : Yao Xiao [États-Unis] ; Xuejun He [États-Unis] ; Yemaiza Ojeda-Lassalle [États-Unis] ; Charleson Poovaiah [États-Unis, Nouvelle-Zélande] ; Heather D. Coleman [États-Unis]Source :
- Biotechnology for biofuels [ 1754-6834 ] ; 2018.
Abstract
Background
Expression of glycosyl hydrolases in lignocellulosic biomass has been proposed as an alternative to improve efficiency of cellulosic ethanol production.
Results
In this study, transgenic hybrid poplar (
Conclusions
Overexpression of hyperthermophilic endoglucanase in feedstock had impacts on plant growth and cell wall composition, especially when the enzyme was highly expressed. Improved glucan saccharification efficiencies from transgenic lines before and after heat treatment could reduce both the economic and environmental costs associated with ethanol production from lignocellulosic biomass.
DOI: 10.1186/s13068-018-1224-7
PubMed: 30147748
PubMed Central: PMC6094567
Affiliations:
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Coleman</name><affiliation wicri:level="2"><nlm:affiliation>1Biology Department, Syracuse University, Syracuse, NY 13244 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>1Biology Department, Syracuse University, Syracuse</wicri:cityArea></affiliation></author></analytic><series><title level="j">Biotechnology for biofuels</title><idno type="ISSN">1754-6834</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>Background</b></p><p>Expression of glycosyl hydrolases in lignocellulosic biomass has been proposed as an alternative to improve efficiency of cellulosic ethanol production. </p></div><div type="abstract" xml:lang="en"><p><b>Results</b></p><p>In this study, transgenic hybrid poplar (</p></div><div type="abstract" xml:lang="en"><p><b>Conclusions</b></p><p>Overexpression of hyperthermophilic endoglucanase in feedstock had impacts on plant growth and cell wall composition, especially when the enzyme was highly expressed. Improved glucan saccharification efficiencies from transgenic lines before and after heat treatment could reduce both the economic and environmental costs associated with ethanol production from lignocellulosic biomass.</p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30147748</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1754-6834</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><PubDate><Year>2018</Year></PubDate></JournalIssue><Title>Biotechnology for biofuels</Title><ISOAbbreviation>Biotechnol Biofuels</ISOAbbreviation></Journal><ArticleTitle>Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility.</ArticleTitle><Pagination><MedlinePgn>225</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13068-018-1224-7</ELocationID><Abstract><AbstractText Label="Background" NlmCategory="UNASSIGNED">Expression of glycosyl hydrolases in lignocellulosic biomass has been proposed as an alternative to improve efficiency of cellulosic ethanol production. <i>In planta</i> production of hyperthermophilic hydrolytic enzymes could prevent the detrimental effects often seen resulting from the expression of recombinant mesophilic enzymes to plant hosts. Utilizing lignocellulosic feedstocks to produce hyperthermophilic hydrolases provides additional benefits for ethanol production in the way of transgenic feedstocks serving as both enzyme providers and cellulosic substrates.</AbstractText><AbstractText Label="Results" NlmCategory="UNASSIGNED">In this study, transgenic hybrid poplar (<i>Populus alba </i>× <i>grandidentata</i>) was generated to express a hyperthermophilic endoglucanase from <i>Thermotoga neapolitana</i> with an optimal temperature over 100 °C. Functional hyperthermoactive endoglucanase was successfully produced in the transgenic events, and altered phenotypic growth was observed in transgenic lines. Moreover, the line with the highest <i>Tn</i>CelB expression in both leaf and developing xylem had reduced lignin content and cellulose crystallinity, resulting in a more digestible cell wall. The activation of <i>Tn</i>CelB by a post-harvest heat treatment resulted in enhanced saccharification efficiencies of transgenic poplar lines with moderate <i>Tn</i>CelB expression and without alteration of cellulose and lignin when not heat-treated. <i>In planta</i> high-level overexpression of a hyperthermophilic endoglucanase paired with heat treatment following harvest, resulted in biomass that was comparable with wild-type lines that underwent a traditional pretreatment for saccharification.</AbstractText><AbstractText Label="Conclusions" NlmCategory="UNASSIGNED">Overexpression of hyperthermophilic endoglucanase in feedstock had impacts on plant growth and cell wall composition, especially when the enzyme was highly expressed. Improved glucan saccharification efficiencies from transgenic lines before and after heat treatment could reduce both the economic and environmental costs associated with ethanol production from lignocellulosic biomass.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Xiao</LastName><ForeName>Yao</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>1Biology Department, Syracuse University, Syracuse, NY 13244 USA.</Affiliation><Identifier Source="ISNI">0000 0001 2189 1568</Identifier><Identifier Source="GRID">grid.264484.8</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Xuejun</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>1Biology Department, Syracuse University, Syracuse, NY 13244 USA.</Affiliation><Identifier Source="ISNI">0000 0001 2189 1568</Identifier><Identifier Source="GRID">grid.264484.8</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ojeda-Lassalle</LastName><ForeName>Yemaiza</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>1Biology Department, Syracuse University, Syracuse, NY 13244 USA.</Affiliation><Identifier Source="ISNI">0000 0001 2189 1568</Identifier><Identifier Source="GRID">grid.264484.8</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Poovaiah</LastName><ForeName>Charleson</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>1Biology Department, Syracuse University, Syracuse, NY 13244 USA.</Affiliation><Identifier Source="ISNI">0000 0001 2189 1568</Identifier><Identifier Source="GRID">grid.264484.8</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>2Present Address: Scion, Te Papa Tipu Innovation Park, 49 Sala Street, Rotorua, 3010 New Zealand.</Affiliation><Identifier Source="ISNI">0000 0004 1936 9203</Identifier><Identifier Source="GRID">grid.457328.f</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Coleman</LastName><ForeName>Heather D</ForeName><Initials>HD</Initials><Identifier Source="ORCID">0000-0002-4923-601X</Identifier><AffiliationInfo><Affiliation>1Biology Department, Syracuse University, Syracuse, NY 13244 USA.</Affiliation><Identifier Source="ISNI">0000 0001 2189 1568</Identifier><Identifier Source="GRID">grid.264484.8</Identifier></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>P41 GM103485</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>08</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biotechnol 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Coleman</name><name sortKey="He, Xuejun" sort="He, Xuejun" uniqKey="He X" first="Xuejun" last="He">Xuejun He</name><name sortKey="Ojeda Lassalle, Yemaiza" sort="Ojeda Lassalle, Yemaiza" uniqKey="Ojeda Lassalle Y" first="Yemaiza" last="Ojeda-Lassalle">Yemaiza Ojeda-Lassalle</name><name sortKey="Poovaiah, Charleson" sort="Poovaiah, Charleson" uniqKey="Poovaiah C" first="Charleson" last="Poovaiah">Charleson Poovaiah</name></country><country name="Nouvelle-Zélande"><noRegion><name sortKey="Poovaiah, Charleson" sort="Poovaiah, Charleson" uniqKey="Poovaiah C" first="Charleson" last="Poovaiah">Charleson Poovaiah</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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